ABSTRACT The objective was to present the floristic survey of the bryophytes of the Parque Estadual da Serra do Brigadeiro. It included an evaluation of the species richness, as well as information about the substrate preference of each species and its geographic distributions in Brazil. We found 208 taxa of bryophytes: one hornwort, 88 liverworts and 119 mosses. This richness represents ca. 26% of the bryoflora of Minas Gerais State and ca. 13% of that known for Brazil. Eighteen of the bryophytes species are endemic to Brazil, whereas 19 species are recorded for the first time in Minas Gerais. The bryoflora showed preference for tree bark (30%) and rock (23%) as substrates. Our data show that the Parque Estadual da Serra do Brigadeiro is an important area for the diversity conservation of Brazilian bryophytes and maintenance of endemic species of the country.