The current study describes the production of silver nanoparticles (AgNPs) to treat Candida glabrata infections. The method involved incubation of silver nitrate (AgNO3) with Aspergillus terreus using a green and straightforward route. The production of AgNPs was confirmed through a color change from transparent yellow to brown as well as by ultraviolet-visible (UV-VIS) spectroscopy. The surface morphology of AgNPs was assessed using a scanning electron microscope. The antifungal activity of AgNPs against C. glabrata was investigated in the serum of 20 infected mice. The mice were divided into four groups, and the level of cytokines: IL-4 and IFN-γ were examined after 21 days. The atomic force microscopy confirmed that the average diameter of AgNPs was 25.1 nm, which is appropriate for delivering silver nanoparticles to treat animals' infection. The concentration of cytokines IL-4 and IFN-γ were significantly (P < 0.05) higher in the C. glabrata-infected group than in the control group. While the cytokines level remained close to average concentration in mice administrated with AgNPs, such a result was comparable with the fourth group of mice (Candida-treated Aspergillus) after treatment with AgNPs.