To determine the presence and quantify the concentrations of aflatoxin (B1, B2, G1 and G2) contained in egg-laying hens commercialized at the Bazurto Market of the Cartagena city (Colombia), seeking to know if levels found were high when their values were compared to this recommended by Colombia in the maximum level. 6 eggs, from randomly chosen main food market of the city and 10 days provided, were analyzed. The analytical technique of high pressure liquid chromatography (HPLC) was used to determine the content of aflatoxins. The presence of aflatoxins G1 and G2 in some samples of the eggs were detected and amounts remains of aflatoxin B1 and B2 were not found. The amounts of total aflatoxins analyzed were high when their values were compared to this recommended by NTC 3581.