A variety of surgical procedures can be performed for the treatment of gingival recession, including the subepithelial connective tissue graft. Due to the morbidity reported by patients, alternative techniques using biomaterials has been frequently discussed. The objective of this study was to report the clinical follow-up of root coverage with coronally positioned flap associated with Mucograft® (collagen matrix) for 24 months. The fine gingival biotype tends to be delicate, possessing a small zone of keratinized mucosa. This type of thickness should be taken into special consideration since it directly influences in the aesthetics and the emergence profile. The gingival recession heights and the thickness of the tissue were measured initially and after 4, 9, and 24 months. 1 year post-operative, partial root coverage (22.5%) and gain in the thickness (100%) of the gingival tissue was observed. Although the root coverage with a coronally positioned flap associated with Mucograft® for this clinical case was not effective, a significant increase in thickness of the gingival tissue that received the collagen matrix was observed; thus, it was considered a viable treatment option and suitable for soft tissue augmentation. This clinical case, the Mucograft® was effective for the thickness of the gingival tissue; thus it is considered a viable treatment option and suitable for periodontal tissue augmentation.