The non-leaf-cutting fungus-growing ants deposited in two entomological collections in Colombia were curated and identified to assess their diversity in the country. We examined 680 specimens, identifying 41 species belonging to seven genera, bringing the total of fungus-growing ant species known from Colombia to 85. The following species are new records for Colombia: Apterostigma angustum Lattke, 1997, Mycetomoellerius jamaicensis (André, 1893), Paratrachymyrmex diversus (Mann, 1916), and Paratrachymyrmex phaleratus (Wheeler, 1925).