Abstract Objective To identify patterns of resistance against various antibiotics in Enterococcus faecalis in urinary tract infections in a population of the Toluca valley, Mexico Methods 155 samples were collected from patients with suspected urinary tract infection without exclusion criteria such as age or gender. Automated equipment was used for the identification of the etiological agent and sensitivity tests. Results 80 positive cultures were obtained, of which 20 strains belong to Enterococcus faecalis, which show 100% sensitivity for penicillins, linezolid, vancomycin, bacitracin, a high pattern of sensitivity for quinolones, and a high pattern of resistance to rifampicin, erythromycin and 100% resistance in tetracycline Conclusion It shows 100% sensitivity to penicillins, vancomycin and linezolid, first-line treatments and for cases of infection complicated by Enterococci. And 100% resistance for tetracycline and high resistance patterns for erythromycin and rifampin.