Abstract Chitosan is a biopolymer extracted from the shell of crustaceans. It is currently possible that this be modified to improve its bacterial properties. In this context, in the present work new materials were synthesized as electro-spun films, where the best conditions were establish that led to biocidal activity against to Staphylococcus aureus ATCC 6538. The addition of PVA to chitosan improves electrospun conditions since the chitosan alone cannot stabilize the voltage. The best interaction with water was resolved at 16 % PVA, increasing the hydrophobic capacity of the film. The ion metallic interacts with chitosan matrix through acetyl group, and the alcohol and ammine group do not participate in electronic interaction. Finally, this work shows that the metal ion increases the biocidal properties of chitosan as a thin film.