The balso, guásimo and cadillo are vegetal species which have flocculating properties used in the clarification of cane juice for the production of panela. Its uncontrolled, permanent and inefficient use has caused depletion in the areas where cane is cultivated and panela is being produced, having a negative environmental impact and an increase in production costs for producers. The present study evaluated the efficiency of mixtures of these three natural flocculants, both in powder and in aqueous solution for the clarification of cane juice as an alternative to improve their use. Initially flocculants were obtained individually, from these three mixtures for the powder flocculant and three in aqueous solution with the following relations: balso/guásimo (1,5:1), balso/cadillo (1,5:1), guásimo/cadillo (1:1). Clarification tests in cane juice were carried out at laboratory scale; for the powder mixtures, concentrations of 0.005% and 0.01% were evaluated, for the mixtures in aqueous solution; concentrations of 0.05% and 0.1% were evaluated respectively. Turbidity and total suspended solids were determined from the clarified samples, It was determined that the mixtures with the best behavior in the clarification process were in guásimo/cadillo aqueous solution (1:1) at a concentration of 0.1%, balso/guasimo (1,5:1) at a concentration of 0.1% and guásimo/cadillo (1:1) at a concentration of 0.05%, presenting 97.27%, 97.16% and 95.31% in removal of total suspended solids present in the cane juice.