Bacterial resistance is a public health problem worldwide whose proper management requires knowledge of its presence and its behavior in each region and country.A survey of the medical literature was conducted to identify levels of resistance to antibiotic markers in Gram positive bacterial isolates from Colombian hospitals.A systematic review of the literature included articles indexed in MEDLINE and LILACS. A manual search was made of Colombian scientific journals and other infectious disease literature not available electronically.A total of 34 observational studies were located, including a series of consecutive reports initiated in 2001. Most of the reports came from the city of Bogota. The rate of methicillin resistance for Staphylococcus aureus and coagulase-negative staphylococci in non intensive care unit isolates ranged between 35%-50% and 72%-76%, respectively. Resistance in intensive care unit isolates had a range between 35%-71% and 74%-83%, respectively. The rate of vancomycin-resistant Enterococcus faecium averaged less than 20% over the years but with large annual variation .Resistance markers appeared in high frequency among Gram positive isolates identified in hospitals in major Colombian cities.