This paper presents a novel non-invasive methodology to study the cutaneous wound healing process in rabbits. Spectral images were used to establish a quantitative evaluation of the healing process time-line dynamics. Measurements were conducted by image analysis of the wounds of an experimental population with two different systems: an acousto-optical acquiring/processing system permitted the experimental quantitative study of different stages naturally occurring during the healing process time-line. Honey and saline solution application were used in a subgroup of the population under study as examples of treatments that could be employed to purportedly accelerate cutaneous tissue restoration, and their efficacy was evaluated. The methodology described on this paper allows for a non-invasive and effective way to quantitatively study/monitor the wound healing processes in terms of spectral changes of the wound area. Finally, it was found that this methodology allows quantitative parametrization of measurements to be used as accurate testing grounds for new wound healing assisting clinical treatments.