Successfully developing a new product, service, or production process for commercial purposes involves complex and dynamic changes, and therefore requires an organizational climate that fosters innovation. In this sense, the means by which knowledge is created and exploited in the firm constitute its key competence and the source of its innovation capabilities. The research objective was to establish the relationship between organizational climate and innovation capability. The sample consisted of 102 Colombian new technology-based firms. Partial least squares structural equation modeling (PLS-SEM) and PLSpredict were used for the statistical analysis. The results indicate that the organizational climate positively and directly influences the innovation capability (organizational ambidexterity, exploration, and exploitation). The findings obtained will help firms, especially those that require a lot of knowledge and carry out their activities in dynamic environments, to understand how organizational climate influences innovation capability, which is understood through the dimensions of exploration, exploitation, and organizational ambidexterity. This will provide new technology-based firms with a higher capacity to adapt to the conditions of uncertainty and complexity of the environment.