Zloza and Al-Harthi reported that the expression of invariant and non-invariant NKT markers was most prominent in the CD4 bright CD8 dim subpopulation [5].In one study, up to 29% of cells were found as invariant CD3+6B11+NKT cells and up to 26% as non-invariant CD3+CD16/56+NKT cells [4].It was also stated that the combination of cell populations not sharing similar features, expressing differentiation and activation of surface markers, and not consuming contaminating cell populations such as NKT cells would mask CD4+CD8+ T-cell subpopulation analyses [5].