Lophostoma brasiliense Peters, 1867 has a wide distribution in the Neotropical region, with records extending from southern Mexico to central Paraguay and Brazil. In Colombia the distribution of this species seems to be discontinuous, due to a scarcity of records, which also makes it difficult to know about its threats and conservation status. Here, we report three new localities from Amazonia, Andean and Orinoquia regions. We updated the distribution and performed ecological niche modelling that suggest a wider presence of this species on the Caribbean and the Orinoquia region. Finally, we added some ecological notes about this species.