This chapter presents the monitoring and research of groundwater resources and natural recharge in the Doñana Natural Area. The subchapter “Monitoring networks for Groundwater Resources and Recent Management Issues of the Doñana” deals with the history and current state of the official monitoring networks for piezometry and hydrochemistry. In the second subchapter “Application of Models in Recent Management and Forecasting of Resources” the current research and the model based water management issues are presented. The third subchapter “Main Knowledge existing on Groundwater Natural Chemical Composition and Contaminant Processes in the Eolian Mantle Aquifer Sector of the Doñana (SW Spain)” provides an overview of hydrochemical research carried out in the eolian part of the aquifer. Subchapter 4 “Active Monitoring Network for Recharge Measurements in the Doñana National Park” presents the current monitoring network for groundwater recharge.