Abstract A chimeric peptide derived from Lactoferricin B (LfcinB) and Buforin II (BFII) was designed. It specifically contains the reported antimicrobial minimal motif sequences LfcinB (20–25): RRWQWR and BFII (32–35): RLLR. The chimeric peptide was synthesized by intramolecular cyclization with a triazole bridge. The Chimera 3 exhibited higher antimicrobial activity against E. coli ATCC 25922 (MIC 93 μM) and S. aureus ATCC 25923 (93 μM) than the original sequences. Furthermore, it did not exhibit a significant hemolytic effect.