This applied research work identifies the impact of the project management offices (PMO) on knowledge management among 502 organizations from Colombian enterprises. First, a literature review about PMOs and knowledge management within the project perspective was conducted. Then, a bibliometric analysis was conducted in order to establish the link between this type of offices and the knowledge management on scientific publications about project management. Afterwards, a comprehensive theoretical framework about the matter of study was build. Subsequently, an information gathering instrument was developed in order to collect data for the research. Next, a nation-wide fieldwork was undertaken by analyzing the organizations. Successively, a detailed statistical analysis was conducted in order to identify the impact of the project management offices on knowledge management within the objects of study. Finally, results and conclusions were documented, and future lines of research were established.