Five new species and one new genus of the tribe Anacolini (Cerambycidae: Prioninae) are described from South America: Motilon hirsutumidusgen. et sp. nov. (Colombia), Oideterus tibiaprocerussp. nov. (Colombia), O. latithoraxsp. nov. (Colombia), and O. nigrumsp. nov. (Colombia), and Otheostethus impostor (Colombia and Ecuador). The key to the American genera of Anacolini is updated. Additionally, data on male morphology of Myzomorphus amabilis (Tippmann, 1960) is provided for the first time. Moreover, interesting color variabilities of Oideterus dichotomus (Galileo, 1987) and O. biraiGalileo et al. 2018 are described. Finally, Oideterus dichotomus is reported from Colombia for the first time.