Creole potato varieties represent an alternative for the agroindustrial chain and an opportunity to contribute to food security and conservation of biodiversity. The content of glucose in the tubers is related to quality and nutritional factors in processed products such as potato chip chips. In order to determine the influence of the genotype - environment interaction on the glucose content in four Creole potato varieties, they undergo different fertilization conditions in four different localities in the Department of Nariño in Colombia. It is found that there is a locality that significantly influences one of the varieties, Botana for the genotype UdenarST Cr42, while for the other varieties there are no significant differences with the design factors.