The objective of this work was to evaluate the potential of digital image analysis based on color (DIAC), using surface color index defined for RGB space, for the determination of total protein content (TPC) by Biuret method.Approximation of surface color index was used to describe quantitatively the color intensity in the digital image of samples and standards.It was concluded that DIAC is a fast and simple analytical alternative for the determination of TPC.Detection and quantification limits were 0.02 mg/mL and 0.08 mg/mL, respectively.In addition, measurements of absorbance can be satisfactorily correlated with measurements of surface color intensity (R 2 = 0.9957).In addition, the importance of control of internal brightness in the measuring device was evidenced from distribution of values of color intensity into the images.It was concluded that DIAC is a RGB model Color intensityDigital image Total protein content