In this paper we present a review of the oldest lithic assemblages of Tibitó 1, El Abra 2, and El Abra 3 (Sabana de Bogotá, Eastern Cordillera), among the oldest and most significant sites in Colombia. The contribution of use-wear analysis on a controlled sample of materials confirms and adds new data to our previous technological review Muttillo et al. (2017. “Revisiting the Oldest Known Lithic Assemblages of Colombia: A Review of Data from El Abra and Tibitó (Cundiboyacense Plateau, Eastern Cordillera, Colombia).” Journal of Archaeological Science: Reports 13: 455–465). The results highlight some critical points and discrepancies with previous studies and interpretations.