In this paper, cone-shaped optical fibers as point light sources of spherical waves for digital lensless holographic microscopy (DLHM) are developed and evaluated. The resolution characterization of DLHM with cone-shaped fibers was carried out and a comparative analysis with respect to the pinhole illumination system was developed. The results are very encouraging and support the viability of these cone-shape fibers as a technological alternative to the pinhole illumination system for DLHM.