The objective of this work was to bring the students closer to a real and three-dimensional structure of the matter, relating this characteristic with the physical and chemical properties of stereoisomeric molecules with chiral centers and checking the didactic transposition through molecular models made in origami. For the methodological development of the research, 101 Colombian students from the Pedagogical and Technological University from Colombia and the University from Boyacá participated, from the academic programs in Chemistry, Biology, Bachelor of Natural Sciences and Environmental Education and Physiotherapy. The results obtained show that the implementation of molecular models in origami strengthens visuospatial skills; in addition, the conceptual appropriation of stereoisomers and molecular representations.