Sensation seeking has been proposed as a risk factor for gambling and gambling problems; however, existing evidence for a relationship between sensation seeking and gambling behaviors is inconclusive and data are lacking for emerging adults and racial/ethnic minorities.In this longitudinal study, we explored the association between developmental trajectories of sensation seeking in childhood/adolescence and gambling and gambling problems in early adulthood in individuals of Puerto Rican origin.Data were collected during 2014-2018 from a subsample of participants in the Boricua Youth Study who were recruited in the South Bronx, New York City and in San Juan and Caguas, Puerto Rico.Sensation seeking was measured using a 10-item instrument modified from the scale created by Russo et al. (1991) for use in children as young as 5 years old.Developmental trajectories of age-adjusted sensation seeking were created using growth mixture models.Gambling and gambling problems were assessed based on the Canadian Adolescent Gambling Inventory (CAGI) version 1.09.Data were analyzed using descriptive methods and multivariable logistic regression.Individuals in the high sensation-seeking class had lower adjusted odds of past-year gambling (OR: 0.36 [0.14, 0.92]) than those in the normative sensation-seeking class while no differences were observed for individuals in the low and accelerated classes.No relationship was found between sensation seeking and past-year gambling problems.Given the severe consequences of early initiation of gambling and gambling problems, other early-life risk factors and alternative hypotheses for the elevated prevalence of gambling problems in young adults and racial/ethnic minority populations should be explored.