Introduction: According to existing data in the literature, patients with ulcerative colitis are a group with higher rates of tuberculosis infection (respect for the general population), including the latency, with a higher risk of progression to active disease. In our country the epidemiological evidence showing an increase in global prevalence in the coming years, whose impact on the epidemiological. The main objective of this report is to study the prevalence of the Latent Tuberculosis Infection (I.L.T.) in a population with Ulcerative Colitis in a Colombian reference center of inflammatory bowel Disease (IBD). Methods: Transversal study. We have analyzed 112 patients of the Partenon Clinic. The study began in February 2014 and ended in March 2015. The analyzed variables have been age, sex, UC time of diagnosed, Severity, treatment. The tuberculin skin test (TST) was performed, with induration of ≥ 5 mm being regarded as positive, considering the pharmacological immunosuppression risk. Results: The prevalence of the observed latent tuberculosis infection has been of 27.67% (n: 31). No significant differences have been observed between type of treatment, sex and severity. Time of diagnosis not shows differences between groups. Conclusion: The importance of screening for latent TB in our population with UC is very important not only before starting anti-TNF therapy, as they will be exposed to immunosuppressive therapy leading to increased risk of reactivation of latent tuberculosis with potential deleterious outcomes.