The plants belonging to the genus Solanum are known for their broad spectrum of biological activity. Therefore, the objective of this work was to evaluate the antibacterial activity of ethanolic and methanolic extracts of S. dolichosepalum against bacterial strains S. aureus, Salmonella spp, P. aeruginosa, and A. hydrophila. The methanolic and ethanolic extracts were obtained by solid-liquid extraction in soxhlet equipment, with subsequent concentration by rotary evaporation. To determine the antibacterial activity, the disc diffusion method was used, using Mueller Hinton agar, Chloramphenicol (30 mg sensitives) as a positive control, and extraction solvents as negative controls. The methanolic and ethanolic extracts of S. dolichosepalum showed a slight inhibitory effect against S. aureus, Salmonella spp, A. hydrophila, and P. aeruginosa, but it was not enough to be considered significant showing resistance to them. The two types of extracts used, ethanolic was the most active against S. aureus, Salmonella spp, A. hydrophila, and methanolic against P. aeruginosa.