Pulp revascularization is an alternative treatment in immature traumatized teeth with necrotic pulp.However, this procedure has not been reported in horizontal root fractures.This is a case report of a 9-year-old patient with multiple horizontal root fractures in 2 upper central incisors that were successfully treated with pulp revascularization.The patient presented for treatment 2 years after the initial trauma, and revascularization was attempted after the initial treatment with calcium hydroxide had failed.Prior to pulp revascularization, cone-beam computed tomography and autoradiograms demonstrated multiple horizontal fractures in the middle and apical thirds of the roots of the 2 affected teeth.Revascularization was performed in both teeth; platelet-rich plasma (PRP) was used in one tooth (#11) and the conventional method (blood clot) was used in the other tooth (#21).Clinical and radiographic follow-up over 4 years demonstrated pulp calcification in the PRP-treated tooth.Neither of the 2 teeth were lost, and the root canal calcification of tooth #11 was greater than that of tooth #21.This case suggests that PRP-based pulp revascularization may be an alternative for horizontal root fractures.