We investigated whether the influence of LMX differentiation (the extent to which the quality of the leader-member relationships within a work unit varies) on work unit commitment is mediated by support climate. We tested this mediated relationship in a sample composed of 30 health care units. The results obtained showed that LMX differentiation measured at Time 1 was negatively related to work unit support climate measured one year later (Time 2), which in turn was positively related to work unit commitment measured at Time 2. The negative indirect effect of LMX differentiation on work unit commitment through support climate was statistically significant. Our study contributes to having a better understanding of the role of LMX differentiation in work unit functioning.