PURPOSE: Women's football has begun to be considered as a potential sport thanks to the performance of sportswomen. For the prevention of injury, the eccentric training appears as part of sports training programs. However, preventive programs do not recognize the characteristics of women, this being the object of this study. Objective. Determine the effects of an eccentric training on the balance and explosive strength in female soccer players from two universities in Bogotá, Colombia. METHODS: Randomized controlled trial, which evaluated the explosive force with the Sargent test and the balance with the Star Excursion Balance Test (SEBT) with the participation of 19 university athletes. The players were randomly distributed among the control group (n = 9) that made the protocol FIFA 11+ and experimental group (n = 10) that made the eccentric exercise protocol. Statistical analysis was performed with SPSS, using comparative statistics between groups like the Student's T test for related samples. RESULTS: Significant changes are shown for the balance variables (p ≤ 0.05), in 15 of the 16 directions, and significant changes in the explosive force (p ≤ 0.05; p = 0.000) of athletes who carried out the training eccentric after 4 weeks of intervention. For the FIFA 11+ group only significant changes were observed (p ≤ 0.05) in 3 of the 16 directions of the SEBT and there were no changes in the Sargent test (p ≥ 0.05, p = 0.141). Therefore, it is verified that the intervention protocol of eccentric training has significant effects on the explosive force, in four weeks of intervention. CONCLUSIONS: This study provides evidence on the use of eccentric protocols in amateur soccer players, showing significant changes in the balance and explosive strength, after 4 weeks of training, giving sustenance to introduce eccentric exercises in warm-up programs and injury prevention.