Urbanization is currently one the most important causes of biodiversity loss. The Colombian Andes is a well-known hotspot for biodiversity, however, it also exhibit high levels of urbanization, making it a useful site to document how species assemblages respond to habitat transformation. To do this, we compared the structure and composition of bird assemblages between rural and urban habitats in Armenia, a medium sized city located in the Central Andes of Colombia. In addition, we examined the influence of urban characteristics on bird species diversity within the city of Armenia. From September 2016 to February 2017 we performed avian surveys in 76 cells (250 x 250 m each) embedded within Armenia city limits; and in 23 cells (250 x 250 m each) in rural areas around Armenia. We found that bird diversity was significantly lower in urban habitats than in rural habitats, and differed in species composition by 29%. In urban cells, with higher abiotic noise intensity and higher impervious surface area, we found lower bird diversity than that in urban cells with higher guadual (Guadua angustifolia patches), and forested surface areas. We did not find segregation of urban cells according to the species composition, although additional bird surveys inside urban forests remnant are needed to be more conclusive about this aspect. Altogether, our results highlight the importance of green areas embedded within cities to conserve bird diversity through reducing the ecological impact of urbanization on avian biodiversity.