Currently, there is a high dependence in border areas of Colombia for the constant supply of petroleum fuels. The biofuels generation from low-cost biological fat materials, easy acquisition and minimizing environmental impacts, are research topics. The objective of this work, was to evaluate the physicochemical characteristics of biodiesel derived from chicken fat, which is managed as agroindustrial waste of the transformation process in the poultry farms of the Sabana Sub Region, Nariño Department, Colombia. Chicken fat oil was evaluated to extraction, purification and transesterification processes by means of basic catalysis, using sodium hydroxide in an alcoholic phase of methanol. The results of the chicken fat biodiesel analysis comply with the stipulations of NTC 5444, with the exception of the viscosity parameter (7.5 mm2/s at 40 ºC), which is higher than the one established in the rule. Chicken fat is an alternative raw material in the production of conventional biodiesel in the same production areas, which meets the specifications of Colombian regulations according to NTC 5444, and could be used in any diesel combustion engine, with benefits like a lower sulfur content and contributing to the research for sustainability.