The purpose of this study was to assess the psychometric properties of a questionnaire on motivation self-regulation strategies in university students. The purposive sample used comprised 315 college students from 2 private universities located in Cali, Colombia. The participants' average age was 19; 51% were women and 49% were men. The original instrument in English (Wolters & Benzon, 2013) was translated into Spanish and underwent exploratory and confirmatory factor analyses to evaluate its construct validity. Results indicate that the adapted instrument also displays adequate indices of convergent and discriminant validity, assessed through correlations with the Motivated Strategies for Learning Questionnaire (Pintrich, Smith, Garcia & McKeachie, 1993), and internal consistency, to assess 5 of the 6 motivation self-regulation strategies considered in the original instrument. The final Spanish version of the instrument is a useful tool for conducting further research on this topic. Further studies should select larger samples of college university students attending both public and private universities while also conducting analyses of the instrument's predictive validity.