Knowledge management is currently considered a key factor for organizational development, even more so with the constant competitive dynamics of companies, which see in knowledge a fundamental potential to improve productivity. In this sense, its assessment is key, but considering the culture as a conditioning variable to the development of this procedure nonetheless. Although several studies have addressed the subject, there has been insufficient research into the call center services companies in Colombia. The research results presented herein are part of a qualitative research project with descriptive and correlational studies on knowledge management in BPO companies in the city of Manizales, Colombia. An assessment of the knowledge management categories and organizational culture was made, based on the adaptation of a knowledge management assessment model, of which 312 observations were made. It is concluded that organizational culture is statistically significant for knowledge management.