Variation in activity patterns and habitat use are subject to both environmental factors and interactions with other species. We evaluated the ecological factors that affect habitat use by Leopardus pardalis as well as the effects of the presence of potential prey, Dasyprocta punctata. From an arrangement of 70 camera traps, installed between March 2015 and February 2016, we estimate the detection probability and habitat use for both species with a single season model. The selected models suggest that L. pardalis habitat use (70.4%, IC: 63.2–77.6) varies negatively with forest edge density around 1000 m radius, but no effects resulting from D. punctata presence are reported. Our results suggest the absence of spatial exclusion between both species in the study area and a coexistence facilitated by marked temporal segregation.