The results will be presented according to the methodology developed; therefore, first will be described the results of concentration and bacterial elution process standardization for Escherichia coli DNA extraction from potable water and seawater samples.The second section will aboard the results of the standard curve development process for Escherichia coli quantification by real-time PCR and finally the standardization results of the Salmonella spp.detection method by real-time PCR in drinking water and sea water.