We present the list of amphibians of the department of Tolima, Colombia, based on specimens deposited in the zoological collection of the Tolima University and other collections in the country. We report 93 species of amphibians for the department: 85 Anurans, 5 Caecilians and 3 Urodeles. In addition, we present an analysis of the altitudinal distribution and conservation status of these species. According to the species similarity along the altitudinal gradient in the department, three groups of amphibians can be recognized: one of lowland species (up to 1500 m a. s. l.), another of intermediate species (from 1501 to 2500 m a. s. l.), and a group of highland species (from 2501 m. a. s. l.). Finally, following the information from the International Union for the Conservation of Nature (IUCN), 3 % of the species from the Tolima department are classified as Critically Endangered, 2 % Near Threatened, 16 % Vulnerable, 7 % Endangered, 59 % Least Concern, 6 % Data Deficient and 7 % Not Evaluated. In conclusion, the department of Tolima offers an important geographic area to the amphibian diversity of Colombia, including several Colombian endemic species, species under threat categories and two species restricted to the department.