Taking into account that recently the study of icthyoplankton has become important as an indicator of the reproductive performance of the reofilics fishes, the present investigation studies their distribution in the Sinú River, Colombia, according to the hydraulic variables (area, speed, depth and open surface).18 samplings were carried out between the years 2001 and 2003, utilizing flow meters, spindles and procedures for the quantification of larvae; it is proposed that a methodology to estimate the total load of icthyoplankton considering that the distribution follows the same pattern as the distribution of the average speed of the river; the results indicate that the greater load of icthyoplankton pass by the areas of lesser speed.The estimations of the quantity of icthyoplankton are overestimated when considering an even distribution; hence it is suggested that the proposed method be utilized, in order to quantify the estimation of icthyoplankton that passes by the principal canal of a river.