A study was carried out to establish the prevalence of bacteria associated with infectious bovine mastitis in some milk-producing municipalities in Norte de Santander Department, from which possible species of <em>Staphylococcus</em>, <em>Staphylococcus</em> and Enterobacteria were isolated and identified. One hundred and sixty three samples which were inoculated by depletion in: Blood Agar and EMB Agar were analyzed and incubated at 37°C/24 h. Preliminary identification of the bacteria was done through conventional biochemical tests and RapID™ ONE System and RapID System PLUS STAPH. A total of 207 bacteria were isolated; 187 isolates corresponded to: <em>Staphylococcus aureus</em>, <em>S. capitis</em>, <em>S. epidermidis</em>, <em>S. haemolyticus</em>, <em>S. saprophyticus</em>, <em>S. simulans</em> and <em>S. xylosus</em>. And 20 isolates to: <em>Escherichia coli</em>, <em>Enterobacter agglomerans</em>, <em>Klebsiella pneumoniae</em>, <em>Shigella spp</em>. and <em>Acinetobacter calcoaceticus</em>. <em>Staphylococcus epidermidis</em> showed the highest prevalence for Pamplona, Pamplonita and Toledo with 19.02, 21, 46 and 7.32%, respectively. In Pamplona, the prevalence of <em>Acinetobacter calcoaceticus</em> and <em>Shigella</em> spp. with 0.49%; <em>Klebsiella pneumoniae</em> and <em>Shigella</em> spp. were isolated in Pamplonita (0.49%), in Toledo <em>E. coli</em> and <em>Enterobacter agglomerans</em> (1.95%). The species isolated in this study have been described as causing mastitis in dairy cattle and their presence is related to cleaning conditions and milking practices.