Santa Maria de la Antigua del Darien (1510-1524) was the first Spanish city in the terra firme of America. Archaeological investigations of the years 2013-2014, promoted by the Colombian Institute of Anthropology and the Colombian Ministry of Culture, have identified and delimited the area of the Spanish town. The evidence collected in the surveys has laid the bases for the hypothesis that the city was constituted of two distinct nuclei, corresponding to the indigenous village that was occupied by the hosts of Fernandez de Enciso and Nunez de Balboa in 1510 and the city founded in 1514 by the governor Pedro Arias de Avila. This hypothesis has been confirmed by the results of the excavations of 2015, object of the present article. Through a large stratigraphic excavation the Spanish occupation phase and a former pre-Hispanic phase were clearly recognized.