The aim of this work is detecting glucose concentration based on low-cost electrical impedance spectroscopy (EIS) that works in a frequency range until 65 KHz through sine waves generated by a direct digital synthesizer (DDS). This system uses a pass band filter to reduce offset before a voltage to current converter which drives frequencies toward an electrode. This EIS employs a microcontroller capable of making simultaneous sampling to measure phase shift between two voltage signals. Three Cole-Cole model circuits were used to evaluate system performance. Besides, stainless-steel bipolar electrode was used to measure glucose at different concentrations in saline solutions, three circuits are proposed to model the systems as solution-electrode employing a constant phase element to approximate the polarization problem, a genetic algorithm was used to fit impedance modulus Z and phase . to the models, where a resistor and a RC circuit were the better approximation showing resistance changes due to glucose variations, which means electrical impedance spectroscopy could by one way to glucose detection.