Growing urban expansion results in the alteration of ecological processes (i.e. predation) within trophic networks. Predation on herbivores is known to vary with the size of the area covered in vegetation, successional stage, altitude, and the structure of the predator community, but there are gaps in information regarding how this occurs in urban and suburban environments. The purpose of this study was to determine whether the predation pressure on artificial models of lepidopteran larvae varied with degree of urbanization, type of substrate, and group of predators (birds or arthropods) in Cali, Colombia. Five hundred and eighteen artificial larvae were placed in two areas of the city (urban vs. suburban) and in two types of substrate (leaf vs. stem) for 30 continuous days and with two replications over time. Total predation was measured as the number of models with evidence of attack by predators. The overall incidence of predation was 24.13%, and was significantly higher in the urban area (63.20%) when compared to the suburban area (36.80%). The leaf substrate was attacked significantly more than the stem (60% vs. 40%). The proportion of attacks carried out by birds was significantly higher (74.40%) than that carried out by arthropods (24.80%). Together, these results suggest that the incidence of predation varies with the disturbance caused by urbanization and by the type of substrate in which prey organisms are found. In addition, the study confirms that birds are the main controllers of herbivorous insects in urban environments.