In the present work, the detection of catechol (CC) was studied by using a biosensor of gold screen-printed electrode functionalized with laccase and cysteine as a linker. Cyclic voltammetry (CVs) studies were performed in order to determine the stability range of the biosensor and the redox processes present on the surface of the modified screen-printed gold electrodes (SPGE). CVs studies showed a variation in the response of the electrodes, finding one oxidation signal at 400 mV in presence of catechol, corresponding to the bio-electrocatalytic oxidation-of catechol. The chronoamperometric response of the sensor to different concentration of catechol was measured applying 500 mV, obtaining a linear response of the current in function of the catechol concentration. The linear range of the detection was 0.02 – 2.4 mM, with a sensibility of 3.93 μA μM−1 cm−2 and a detection limit of 1.2 μM with a response time lower than 5 s. The results obtained in this work open new possibilities for the development of portable electrochemical biosensors for quick and efficient detection of catechol in industrial wastewater.