In order to contribute to the knowledge of the chemical constituents and bioactivity of lichens from Colombian páramo region, methanolic extracts of Lobariella pallida and Stereocaulon strictum var. compressum were submitted to phytochemical and cytotoxic studies. Lobariellin (methyl 3-formyl-2,4-dihydroxy-5,6-dimethylbenzoate) (1), along with methyl orsellinate (2) were isolated from the Lobariella pallida. The active constituents namely porphyrilic acid (3), methyl haematommate (4), methyl β-orcinolcaboxylate (5) and atranorin (6) were isolated from the Stereocaulon strictum. The structures of 1-6 were determined by analysis of their spectroscopic (NMR, IR, UV) and spectrometric data. In addition, the structures of 1, 2 and 3 were unequivocally corroborated by single-crystal X-ray diffraction studies. Neither methanol extracts nor compounds 1, 3, 4 and 6 were cytotoxic to the normal (HaCaT) and transformed (MCF-7, HeLa, K562, PC3) mammalian cell tested, which could be indicative of a possible innocuousness and be helpful in other biomedical usefulness.