Objective: To assess in vitro activity of essential oil of Cymbopogon citratus against Fusarium equiseti associated with yam cultivation in the department of Sucre. Materials and Methods: Two municipalities, Corozal and Tolu, each extraction of essential oils (AE) was performed, from dry and fresh leaves were sampled. 1 ppm, 1.000 ppm, 2.000 ppm, 3.000 ppm and 4.000 ppm were tested, and the percent inhibition of the fungal Colonies Forming Units (CFU) was determined. Findings: The concentration of 4.500 ppm for all oils had the highest percentages of inhibition, with a confidence level of 95%. AE Corozal, fresh tossed lower concentrations for MIC and MCF. The chromatographic profile of the four essential oils showed the presence of terpenoids, such as citral, linalool, myrcene and β-Pinene, in which demonstrated antimicrobial and antifungal activity compounds. Application: The use of essential oil of Cymbopogon citratus, is established as an alternative future potential for management and control field tuber rot caused by Fusariumequiseti in growing yams. Keywords: Essential Oil, Inhibition, Phytopathogen, Yam