This paper presents an updated list of soil ants of the Colombian Amazon collected in three different river basins: the Amazon, the Caquetá and the Putumayo. The list includes 10 subfamilies, 60 genera and 218 species collected from TSBF monoliths at four different depths (Litter, 0 - 10 cm, 10 - 20 cm and 20 - 30 cm). This updated list increases considerably the knowledge of edaphic macrofauna of the region, due to the limited published information about soil ant diversity in the Colombian Amazon region. This is the first checklist of soil ant diversity of the Colombian Amazon region. Six new records of species for Colombia are exposed: Acropygatricuspis (LaPolla, 2004), Typhlomyrmexclavicornis (Emery, 1906), Typhlomyrmexmeire (Lacau, Villemant & Delabie, 2004), Cyphomyrmexbicornis (Forel, 1895), Megalomyrmexemeryi (Forel, 1904) and Myrmicocryptaspinosa (Weber, 1937), most of them corresponding to subterranean ants.