Objective: To assess the reliability, sensitivity and specificity of new set of diagnostic criteria, which might allow early detection of, and differentiation between, parasites located in the parenchyma or in the extraparenchymal compartments. Background: The diagnosis of neurocysticercosis (NCC) remains problematic due to the heterogeneity of its clinical, immunological and imaging characteristics Design/Methods: The new set of diagnostic criteria was developed by a group of Latin American experts in NCC by consensus. A multicenter, retrospective study, in accordance with the Standards for Reporting of Diagnostic Accuracy was then conducted. The reference standard for diagnosis of active NCC was the disappearance or reduction of cysts after anthelmintic treatment. Ninety-three cases (with NCC) and 93 controls (without NCC) were selected based on specific inclusion and exclusion criteria. Six independent neurologists blinded to the diagnosis evaluated the cases and controls using the new diagnostic criteria. Mixed-effects logistic regression models were used to estimate sensitivity and specificity Results: The inter-rater reliability (kappa) of diagnosis among evaluators was 0.60. For diagnosis of NCC vs. no NCC, the validation of the new criteria had a sensitivity of 93.2% and specificity of 81.4%. Parenchymal NCC had a sensitivity of 89.8% and specificity of 80.7% and extraparenchymal NCC had a sensitivity of 65.9% and specificity of 94.9%. Conclusions: These criteria are both reliable and valid and represent a new tool for clinical practice and research. An advantage of the new criteria is that it takes the location of parasites, an important factor that determines the clinical, radiological and immunological presentation of the disease, and importantly, the treatment and prognosis of the disease into account. Study Supported by: University of Cuenca, Ecuador Disclosure: Dr. Carpio has nothing to disclose. Dr. Fleury has nothing to disclose. Dr. Romo has nothing to disclose. Dr. Abraham has nothing to disclose. Dr. Fandino has nothing to disclose. Dr. Duran has nothing to disclose.