Objective: We evaluated the association of several single-nucleotide polymorphisms in the triggering receptor expressed on myeloid cells 2 ( TREM2 ) gene in a Colombian sample of late-onset Alzheimer disease (LOAD). Methods: The p.Q33* (rs104894002), p.R47H (rs75932628), p.R62H (rs143332484), and p.D87N (rs142232675) variants of TREM2 gene were directly genotyped using KASPar technology in 358 cases and 329 healthy controls. Sanger sequencing was used to validate >10% of KASPar’s results. The Fisher exact test was used to compare the distribution of allelic and genotype frequency between cases and controls, and the Bonferroni correction was set at P <0.05. Results: The minor allele frequency of rs75932628-T was 0.009 in cases and was not found in any healthy controls which suggests a significant association between rs75932628-T and LOAD risk in our sample ( P =0.010). The rs143332484-T variant did not exhibit a significant association ( P =0.160), whereas rs104894002 and rs142232675 were not found. Conclusions: Our findings suggest that the rs75932628-T variant of TREM2 is an important risk factor for LOAD in the Colombian population.