Objectives: Contribute to the phytochemical study of the leaves of the plant species Muntingia calabura (Muntingiaceae) distributed in Colombia. Methods: The leaves of Muntingia calabura, collected in the municipality of Honda, Tolima -Colombia (Geographic coordinates: 5 ° 11 ′28′ ′N, 74 ° 44′ 34 ″ W) were extracted by cold maceration with 96% ethanol; this extract was fractionated by liquid /liquid partition and chromatographic methods. The structural elucidation of the isolated compounds was carried out by CG-MS and NMR techniques (experiments 1H, 13C, COSY, J-MOD and HSQC). Findings: The phytochemical work developed in Muntingia calabura leaves allowed the isolation and identification of a mixture composed of an oxygenated sesquiterpene and a lignan (α-eudesmol and sesamin), hexatriacontane, scopolin and flavonoid 3, 5, 7, 3′, 4′ Pentahidroxiflavona (quercetin) which are identified for the first time for this species. Keywords: Muntingia calabura, muntingiaceae, Scopolin, Quercetin, GC-MS