The operation stage of an industrial automation project begins with the commissioning process, where all the necessary adjustments are made to ensure the proper performance of the software developed and the devices involved in the automation sequences. This process can be somewhat delicate, since any aspect not foreseen in the software could delay the beginning of the project. In fact, any aspect of software, communication or assembly that is not considered can result in serious losses, given that many times fragile and expensive devices are used. Virtual commissioning has been in recent years a favorable solution to these problems, saving a lot of financial resources for companies wishing to implement automated processes. This paper presents a case study for the automation industry, in which a virtual scenario representing a bottling plant is able to communicate with any kind of PLC (Programmable Logic Controllers) through a communication protocol and a physical interface that work with real digital and analog signals. Both, the communication protocol and the physical interface, are original proposals developed in this work. Key Words: Virtual Commissioning, Industrial, Automation