Background . A screening for malaria parasites was conducted with asymptomatic residents in Colombia. Methods . A descriptive study was carried out in December 2012 in four municipalities of Urabá region in Colombia. A convenience sample of 400 subjects was selected. Participants responded to a survey regarding epidemiological data and blood samples were taken from capillary blood obtained by finger prick for thick smear, rapid diagnostic test (RDT), and polymerase chain reaction (PCR). Results . 399 subjects aged 0.2-98 years were studied (median 22; 221 female (55%)). Episodes of malaria in the last year confirmed by thick film were reported by 47 participants (12%). In 399 samples tested by RDT 4 (1%) were positive (1 with P. falciparum , 3 with P. vivax ), and 3 were confirmed by PCR. In 399 thick blood smears examined 5 (1.3%) were positive (2 with P. falciparum , 3 with P. vivax ), and 3 were confirmed by PCR. In 227 samples, PCR showed 6 (2.6%) positive samples. The parasitaemia was below 1,440 parasites/ μ L. The best agreement between diagnoses was found between the RDT and thick blood smears (Kappa = 0.75). Conclusion . Plasmodial afebrile infection was found in 2% of the studied population, by three diagnostic methods, in residents from a low endemic malaria region in Colombia.