<h3>Background</h3> Traditionally, the role of Rheumatoid Factor (RF) and/or Anti-citrullinated antibodies (ACCP) presence have characterised for diagnosis and prognosis of Rheumatoid Arthritis (RA). However, Anti-nuclear antibodies (ANA) are not routinely measured for the diagnosis of the disease or RA prognosis establishment during first appointment. Recently, evidence showed that positive ANA titles could be considered as poor prognosis factor for RA, and also a higher probability of developing immunogenicity against biologic therapies. <h3>Objectives</h3> To compare the disease activity measurement from a Colombian cohort of patients with RA, based on their auto-antibodies profile. <h3>Methods</h3> The study used a cohort of Colombian patients with RA. A database was developed using the information from the clinical records. The data included were: RF, ACCP, ANA, and the disease activity measured using DAS-28 ESR. Disease activity results were obtained in the following periods of time: 0, 3, 12, 24 and 36 months. Patients were classified based on the different autoantibody profiles (RF/ACCP/ANA:—, +–, -+-, –+, ++-, +-+, -++, +++). Mean DAS-28 ESR results from each period of time were calculated. Also mean weekly Methotrexate (MTX) dose was calculated for each profile. Mean differences between initial, and the follow-up period were calculated using Kruskal-Wallis test. Statistical analysis was made using STATA 12.0 software. <h3>Results</h3> 635 patients with RA were included. 32% of them were men, and 68% were women. Mean age was 54.3 years. The most prevalent profile was +++with 118 patients, and the less frequent was +–. Patients with +++profile had the best response to treatment over time, but also they required more MTX dose. Less response during time was observed with +– profile, however the amount of patients from these group was relatively low. As it was expected,—profile patients required less weekly MTX dose (9.26 mg). It was interesting that patients with –+profile present a worst outcome based on DAS-28 activity, and less response to the treatment. <h3>Conclusions</h3> Results from the study suggest the importance of including the measurement of ANA titles in the initial categorization and follow-up of patients with RA. The presence of ANA seems to have a worst prognosis. ANA co-existence with ACCP appear to have a worst outcome, compared to ++- or +-+profile. Auto-antibody profile in RA could direct the best therapeutic strategy for each patient. Validation of these results are required based on other cohorts. <h3>Reference</h3> [1] Sanmarti R, Gomez-Puerta JA. Biomarcadores en la artritis reumatoide. Reumatol Clin. 2011;6(S3):S25–S28. <h3>Disclosure of Interest</h3> None declared